At the top of this page, you will find a button that unlocks full access to the Medical Laboratory Science (ASCP) practice test. Right below, there’s an opportunity to engage with an extensive practice test tailored for thorough review. These tests are essential tools, crafted not only to highlight your strengths but also to pinpoint areas for enhancement, thereby allowing you to refine your study strategy with precision.

To enhance your preparation further, particularly in areas requiring extra attention, we’ve created specialized quizzes. These quizzes are carefully constructed to focus on distinct aspects of the Medical Laboratory Science (ASCP) exam, offering a focused and effective method of study.

ASCP Medical Laboratory Science Domain Exams

The American Society for Clinical Pathology (ASCP) Board of Certification (BOC) Medical Laboratory Scientist (MLS) exam is a comprehensive and rigorous assessment designed for individuals seeking certification in the field of laboratory medicine. This certification is a benchmark of excellence and is often required for employment in hospitals and clinical laboratories. The MLS exam covers a broad range of disciplines within medical laboratory science. 

Below is a breakdown of the exam content areas:

Domain 1: Blood Banking

  • Percentage of Exam: 19%
  • Focus: Topics include blood group systems, antibody screening and identification, compatibility testing, transfusion therapy, and blood donation processes.

Welcome to your ASCP MLS Domain 1: Blood Banking

Domain 2: Urinalysis and Body Fluids

  • Percentage of Exam: 8%
  • Focus: Covers the physical, chemical, and microscopic examination of urine, cerebrospinal fluid, and other body fluids. This section also includes the clinical significance of test results.

Welcome to your ASCP MLS Domain 2: Urinalysis and Body Fluids

Domain 3: Chemistry

  • Percentage of Exam: 19%
  • Focus: Encompasses clinical chemistry analyses, principles, and applications of instruments, laboratory mathematics, and quality control. Topics also cover endocrinology, toxicology, and therapeutic drug monitoring.

Welcome to your ASCP MLS Domain 3: Chemistry

Domain 4: Hematology

  • Percentage of Exam: 19%
  • Focus: Covers the formation and function of blood cells, hemostasis, and disorders of the blood and bone marrow. It includes laboratory evaluation of blood cells, hematological testing methodologies, and quality control in the hematology laboratory.

Welcome to your ASCP MLS Domain 4: Hematology

Domain 5: Immunology

  • Percentage of Exam: 8%
  • Focus: Involves the study of immune responses, principles of antigen-antibody reactions, immunologic disorders, and laboratory techniques in serological testing.

Welcome to your ASCP MLS Domain 5: Immunology

Domain 6: Microbiology

  • Percentage of Exam: 19%
  • Focus: Encompasses bacteriology, virology, mycology, parasitology, and the principles of microbial pathogenesis. It includes the collection, transport, and processing of microbiological specimens, microbial culture techniques, and antimicrobial susceptibility testing.

Welcome to your ASCP MLS Domain 6: Microbiology

Domain 7: Laboratory Operations

  • Percentage of Exam: 8%
  • Focus: Includes laboratory safety, quality assurance and quality control, laboratory information systems, and management practices. This section is crucial for understanding the operational aspects of running a clinical laboratory.

Welcome to your ASCP MLS Domain 7: Laboratory Operations

Exam Domain% of ExamFocus
Blood Banking / Immunohematology19%Topics include blood group systems, antibody screening and identification, compatibility testing, transfusion therapy, and blood donation processes.
Urinalysis and Other Body Fluids8%Covers the physical, chemical, and microscopic examination of urine, cerebrospinal fluid, and other body fluids.
Chemistry19%Encompasses clinical chemistry analyses, principles and applications of instruments, laboratory mathematics, and quality control.
Hematology19%Covers the formation and function of blood cells, hemostasis, and disorders of the blood and bone marrow.
Immunology / Serology8%Involves the study of immune responses, principles of antigen-antibody reactions, immunologic disorders, and laboratory techniques in serological testing.
Microbiology19%Encompasses bacteriology, virology, mycology, parasitology, and the principles of microbial pathogenesis.
Laboratory Operations8%Includes laboratory safety, quality assurance and quality control, laboratory information systems, and management practices.

Important Notes:

  • Total Questions: The exam consists of approximately 100 scored items and 20 pilot (unscored) items, making a total of 120 questions. The exam duration is 2.5 hours.
  • Adaptive Testing: The MLS exam utilizes computer adaptive testing (CAT), which means the difficulty of the exam will adjust based on the test taker’s ability.
  • Preparation and Resources: Preparation for the ASCP BOC MLS exam requires a thorough understanding of the subjects mentioned above. Official ASCP study guides, practice exams, and textbooks covering clinical laboratory science are invaluable resources. Additionally, attending review courses and participating in study groups can enhance understanding and retention of complex concepts.

Medical Laboratory Science (ASCP) Exam Breakdown

The Medical Laboratory Science (ASCP) Exam, also known as the ASCP Board of Certification (BOC) exam, is a certification examination for medical laboratory professionals. The American Society for Clinical Pathology (ASCP) offers this certification to individuals who have met their educational, experience, and examination requirements, signifying a standard of excellence in the field of medical laboratory science.

Breakdown of the ASCP Exam

  • Eligibility Requirements: Candidates must meet specific education and experience criteria. These typically include a bachelor’s degree in medical laboratory science or a related field and completion of a clinical laboratory internship or equivalent experience.
  • Exam Content: The exam covers various areas of medical laboratory science, including clinical chemistry, hematology, immunology, immunohematology (blood banking), microbiology, and laboratory operations. The content distribution varies, with a focus on testing knowledge and problem-solving abilities in real-world scenarios.
  • Format and Duration: The ASCP BOC exam is a computer-based test consisting of multiple-choice questions. Candidates are allotted a certain amount of time to complete the exam, usually several hours.
  • Preparation and Study Resources:
    • ASCP BOC Study Guide: The official ASCP study guide provides detailed information on the exam content, sample questions, and preparation tips.
    • Review Courses: Various organizations and educational institutions offer review courses and workshops designed to help candidates prepare for the exam.
    • Practice Tests: Taking practice tests can help candidates familiarize themselves with the exam format and identify areas where further study is needed.
    • Textbooks and Online Resources: Several textbooks and online resources are recommended by ASCP and other professional bodies for in-depth study.
  • Application and Fees: Candidates must apply to take the exam and pay the associated fees. The application process includes verifying eligibility and selecting a testing location and date.
  • Results and Certification: After passing the exam, candidates receive certification from ASCP. This certification is a mark of professionalism and competence in the medical laboratory science field and may be required by some employers.

How to Become Medical Laboratory Science (ASCP) Certified

To become an ASCP (American Society for Clinical Pathology) certified Medical Laboratory Scientist (MLS), you typically need to follow a series of steps, which involve completing educational requirements, gaining relevant laboratory experience, and passing a certification exam. 

Here’s an overview of the process:

  • Educational Requirements: You must have a bachelor’s degree in medical laboratory science (MLS) or a related field from an accredited program. The ASCP Board of Certification provides a list of accredited programs.
  • Clinical Experience: Before taking the certification exam, you’ll need to complete a clinical laboratory internship through an NAACLS-accredited medical laboratory scientist program. The duration and specifics of these internships can vary.
  • Application for Certification: Once you’ve met the educational and experience requirements, you can apply for the certification exam. This involves submitting your transcripts, proof of clinical experience, and any other required documentation to the ASCP.
  • Studying for the Exam: It’s crucial to prepare for the certification exam thoroughly. The ASCP offers various study resources, including practice exams, study guides, and more.
  • Taking the Exam: The ASCP certification exam is computer-based and offered at Pearson VUE test centers. It covers various areas of laboratory science, including chemistry, hematology, microbiology, and immunology.
  • Maintaining Certification: Once certified, you’ll need to participate in the ASCP’s Certification Maintenance Program (CMP), which involves completing continuing education and professional development activities to maintain your certification.

Our Experience Teaching Medical Laboratory Science (ASCP) Candidates To Pass The Exam 

The journey of creating our Medical Laboratory Science (ASCP) practice tests at Career Employer has been an extraordinary adventure, filled with both accomplishments and hurdles. Driven by the diverse range of topics included in the exam, our aim has always been to meet the wide array of learning preferences of our students.

We’ve meticulously shaped every facet of our approach to guarantee that every student is thoroughly equipped to achieve excellence in the exam. Our dedication to delivering an all-encompassing educational experience is evident in the extensive and detailed nature of our practice materials, underscoring our commitment to the success of our students.

Teaching Medical Laboratory Science (MLS) for the ASCP (American Society for Clinical Pathology) certification can be both a rewarding and challenging experience from a lecturer’s perspective. Here’s an in-depth look at the nuances of such a role:

Curriculum Development

The lecturer plays a critical role in developing a curriculum that not only covers the theoretical aspects of medical laboratory science but also provides comprehensive training on practical skills. This involves staying updated with the latest ASCP guidelines and integrating them into course materials. Resources like the ASCP’s official website, textbooks recommended by the ASCP, and peer-reviewed journals in the field are essential in ensuring the curriculum is up-to-date and comprehensive.

Engaging Teaching Methods

To effectively teach the diverse topics covered in the MLS certification, lecturers must employ a variety of teaching methods. This includes lectures for theoretical knowledge, laboratory sessions for practical skills, and case studies for critical thinking and problem-solving abilities. Tools like virtual labs, interactive simulations, and online platforms such as LabCE (Laboratory Continuing Education) can enhance the learning experience.

Continuous Professional Development

For lecturers, staying abreast of the latest developments in medical laboratory science is crucial. This involves attending seminars, workshops, and continuing education courses. Membership in professional organizations like the ASCP provides access to a wealth of resources, including online courses, webinars, and journals that help educators update their knowledge and teaching methods.

Assessment and Feedback

Creating effective assessments is vital to gauge the students’ understanding and readiness for the ASCP certification exam. These assessments should be diverse, including quizzes, practical exams, and project-based evaluations. Feedback should be constructive, timely, and aimed at helping students improve. Utilizing practice exams and review questions from resources like the ASCP Exam Prep app and BOC Study Guide can be particularly beneficial.

Support and Motivation

The journey to ASCP certification can be rigorous for students. Lecturers must provide not just academic support but also motivation and guidance to help students navigate challenges. Creating a supportive classroom environment, offering mentoring sessions, and encouraging engagement with study groups or online forums can play a significant role in student success.

At Career Employer, our Medical Laboratory Science (ASCP) certification practice tests are developed by a team of experts celebrated for their deep expertise and broad experience in leading candidates to their Certified Medical Laboratory Science (ASCP) exam achievements. This team’s profound understanding of the domain has been instrumental in guiding more than 1000 medical lab technicians to successfully achieve Medical Laboratory Science (ASCP) certification, with a particular emphasis on addressing the specific needs of medical lab technicians.

Our collective is made up of seasoned medical lab technicians, each with over a decade of dedicated practice in medical lab technology. Such extensive experience ensures that our practice materials are not only produced by the leading experts in the field but are also highly effective in preparing candidates for success in their certification pursuits. Our steadfast dedication to excellence means that individuals who engage with our resources are comprehensively prepared to meet and exceed their certification goals, underscoring our commitment to their success.

Effective Study Strategies and Methods

To excel in the Medical Laboratory Science (ASCP) exam, a robust study strategy focusing on understanding rather than rote memorization, consistent practice, and leveraging community support is essential. Here are the most effective study strategies and resources distilled from comprehensive guides and insights from past test-takers:

  • Understand the ASCP Exam Format and Content: Familiarize yourself with the structure of the exam, which typically includes 100 multiple-choice questions to be completed in 2 hours and 30 minutes. These questions cover various topics like clinical chemistry, hematology, microbiology, blood banking, immunology, and laboratory operations. Understanding the Computer Adaptive Testing (CAT) format used in the ASCP exam is also crucial, as the difficulty of questions adapts based on your answers​.
  • Comprehensive Study Materials:
    • Textbooks and Study Guides: Key resources include “The Board of Certification Study Guide — Clinical Laboratory Certification Examinations, Enhanced 6th Edition”, “Clinical Laboratory Science Review”, and “Medical Laboratory Science Review”. These guides align with the exam content and style, providing a solid foundation for study​​.
    • Online Courses and Webinars: Enroll in specific prep courses that offer structured learning paths, webinars, and access to instructors for doubt clarification​.
    • Practice Questions and Mock Exams: Engage with as many practice questions and mock exams as possible. These resources simulate the exam environment and help identify areas of strength and weakness​.
  • Active Study Strategies:
    • Time Management Techniques: Develop a study schedule focusing on different topics or study materials daily or weekly. Prioritize subjects based on their weight in the exam and your comfort level with each topic​.
    • Approaches to Multiple Choice Questions: Practice strategies for approaching multiple-choice questions, such as reading questions carefully, eliminating incorrect choices, and considering all options before making a decision​​.
    • Case Studies and Laboratory Scenarios: Understand and practice critical thinking skills to analyze and solve case studies and laboratory scenarios, which are integral parts of the exam​.
  • Community and Study Groups:
    • Join Study Groups and Forums: Collaborating with peers through study groups or online forums can provide motivation, diverse insights, and shared resources. These communities often conduct mock exams and share study tips, which can be highly beneficial​​.
  • Self-Assessment Tools: Utilize self-assessment tools to gauge your preparation level and understand your strengths and weaknesses. Regular self-assessment can guide your study focus and improve your chances of success​.
  • Specialized Study Guides for MLS ASCP:
    • MLS ASCP Study Guide: Offers a comprehensive syllabus for the Medical Laboratory Scientist certification, detailing subjects such as Blood Banking, Chemistry, Hematology, Immunology, Laboratory Operation, Microbiology, and Urinalysis and Body Fluids. This guide emphasizes the importance of understanding key topics in each area​.
  • Resource Utilization:
    • ASCP BOC Reading List: ASCP provides a recommended reading list that covers all necessary textbooks and online resources across different subjects. Using this list can help ensure you’re studying the most relevant and up-to-date material​​.

By combining these strategies and resources, candidates can enhance their understanding, improve test-taking skills, and significantly increase their chances of passing the Medical Laboratory Science (ASCP) exam. Remember, consistency, active learning, and strategic practice are key to success.


ItemNon-member PriceMember PriceContent/Features
MLS Online Review CourseUSD 59.00USD 49.0030 hours of content, 10 modules, includes exam strategies
BOC Study GuideUSD 99.00USD 89.00Recommended comprehensive resource for exam preparation

The cost associated with obtaining the Medical Laboratory Science (ASCP) board certification encompasses several elements, including application fees, study materials, and potentially additional resources for preparation. 

  • ASCP Board of Certification (BOC): Offers a comprehensive certification process for medical laboratory professionals. The specific exam fees were not detailed in the accessible sources, but ASCP is known for a range of certification exams, with fees varying significantly based on the exam type.
  • MLS Online Review Course:
    • Non-member price: USD 59.00
    • ASCP member price: USD 49.00
    • About 30 hours of content across 10 modules, including a comprehensive review of key exam content areas, case studies, testing process insights, and exam strategies​.
  • Study Materials:
    • BOC Study Guide: USD 99 for non-members, USD 89 for ASCP members. Recommended as a comprehensive preparatory resource alongside the online review course​​.
  • Additional Resources and Preparation Costs: Potential expenses for extra study materials, practice exams, or continuing education courses are not included in the initial cost estimations and can vary widely based on individual needs and choices.

For the most precise and current information regarding exam fees and related costs, direct consultation with the ASCP Board of Certification or visiting their official website is advised.

Reliable Study Materials and Resources

Preparing for the Medical Laboratory Science (ASCP) certification exam can be a daunting task, but numerous online resources can help you study efficiently and effectively. Here’s a list of the best online resources that cover a wide range of study materials, from comprehensive courses to practice tests and review questions:

  • MLSi Academy: Offers a broad selection of preparation courses for various ASCP certification exams, including blood banking, cytogenetics, cytotechnology, histotechnology, and more. Their courses aim to cover all necessary content areas and condense the BOC reading list into comprehensive learning materials​.
  • Mometrix: Provides free practice tests for the Medical Laboratory Scientist certification exam. The site offers a detailed breakdown of the exam content, including areas such as hemostasis, immunology, microbiology, and laboratory operations​.
  • ASCP’s BOC Exam Prep: This is a direct resource from the American Society for Clinical Pathology, offering study guides, interactive practice exams, and review courses. They have bundled packages for the MLS and MLT exams that include study guides, quick compendiums of medical laboratory sciences, and interactive practice exams​.
  • The MLS Online Review Course by ASCP: This review course is designed to help you identify your strengths and weaknesses across various topic areas on the ASCP MLS certification examination. It offers 25-30 hours of interactive content, including microscopic images, test results, real-world case studies, and a comprehensive overview of the certification process.
  • CLS Resource: A website dedicated to basic clinical laboratory science, targeting students preparing for the ASCP or iASCP exam. It offers study and review materials across several domains such as hematology, microbiology, chemistry, blood bank, and urinalysis​​.
  • Pass ASCP: Provides a complete review package with over 3000 questions, flashcards, and mind maps tailored for the ASCP Board of Certification exams. It also offers quizzes to boost confidence as the test date approaches, with options for practice and review mode to facilitate progressive learning​.

Each of these resources provides a unique approach to preparing for the ASCP certification exams. Whether you prefer engaging with interactive content, tackling practice questions, or diving into comprehensive courses, there’s something available to suit your study preferences and help you succeed in obtaining your certification.

Tips for Exam Day

For the Medical Laboratory Science (ASCP) exam, comprehensive preparation and a strategic approach on the exam day are crucial to success. Here’s a consolidated guide based on the best resources available:


  • Comprehensive Content Review: The exam covers various topics such as Blood Group Immunology, Urinalysis and Body Fluids, Chemistry, Hematology, Immunology, Microbiology, and Laboratory Operations. A thorough understanding of these subjects is imperative​​.
  • ASCP MLS Exam Registration: Ensure you’ve properly registered for the exam by creating an ASCP account, submitting the application with the fee, and uploading any required documentation.

Exam Day Tips

  • Arrive Early: Be at the testing center at least 30 minutes before your appointment. This will give you ample time to complete the necessary pre-exam procedures.
  • Valid ID and Biometric Information: You will need to present a valid, government-issued photo ID. The testing center will also collect your signature and biometric information​.
  • Personal Items: You may bring a non-programmable calculator. Other personal items, including cell phones and keys, should be stored in the assigned locker​.
  • Understanding the Exam Format: The MLS exam is computer-adaptive, meaning the difficulty adjusts based on your answers. It’s crucial to aim for accuracy in your responses, especially since the final question’s difficulty level determines pass/fail status​.

Additional Resources

  • MLS ASCP Preparation Course: Enrolling in a preparation course can provide you with a structured study plan, expert instructors, and practice exams. Courses like the one offered by MLSi Academy provide a comprehensive overview, interactive learning, and personalized support​.
  • Practice and Revision: Using platforms like PASSASCP.COM that offer extensive question banks, quizzes, and practice exams can be highly beneficial. These resources are designed to build confidence and ensure you’re well-prepared for the exam format​​.

In conclusion, success in the ASCP MLS exam hinges on thorough preparation, understanding the exam’s structure, and utilizing available resources effectively. Take advantage of practice tests, preparatory courses, and review materials to reinforce your knowledge and boost your confidence.

Post-Exam: Next Steps

After completing the Medical Laboratory Science (ASCP) exam, there are several strategies and next steps you can take to ensure you’re moving forward effectively in your career. Engaging with online resources and forums can provide you with a wealth of information and support.

Post-Exam Strategies

  • Evaluate and Reflect: Take some time to reflect on the exam experience. Think about the areas you felt confident in and those where you faced challenges. This can help guide your ongoing learning and professional development.
  • Stay Updated: The field of medical laboratory science is constantly evolving. Stay informed about the latest research, techniques, and technologies by subscribing to relevant journals and online forums.
  • Engage in Professional Development: Look for continuing education courses, webinars, and workshops. MLSi Academy and other platforms offer a variety of courses that can enhance your skills and knowledge.
  • Join Professional Networks: Participate in forums and social media groups related to Medical Laboratory Science. This can provide opportunities for networking, mentorship, and career advice.

Preparation for Certification and Career Advancement

  • ASCP Certification Preparation: MLSi Academy offers a comprehensive ASCP certification preparation course. This course covers all the necessary content for the exam, including blood banking, chemistry, urinalysis & other body fluids, immunology, hematology, laboratory operations, and microbiology. It provides an in-depth review of the exam content, test-taking techniques, and time management strategies, all accessible online​​.
  • Practice and Review: PASSASCP.COM offers over 3000 questions, flashcards, and mind maps to help you prepare for your ASCP BOC exams. It features on-demand review exams and a package designed to boost confidence with ASCP MLS quizzes. Practicing with these resources can help bolster your confidence and ensure you’re well-prepared for exam day​​.
  • Understanding Certification Types: It’s important to understand the differences between the MLS ASCP certification for U.S. citizens and the MLS ASCPi certification for international professionals. Both certifications share identical curricula and syllabi, ensuring uniformity in examination content and evaluation. Depending on your academic and professional background, you’ll need to choose the certification that aligns with your qualifications​​.
  • Continuous Learning: Beyond certification, the field requires continuous learning and adaptation. Engaging with a professional community through platforms like MLSi Academy can provide ongoing support and education, helping you to stay at the forefront of the field​​.
  • Career Advancement: Certification can open doors to new job opportunities and career advancement. It demonstrates a commitment to the profession and a high level of expertise. Consider exploring specializations within the field to further enhance your career prospects.

Remember, the journey doesn’t end with passing the exam. Continuous learning, networking, and professional development are key to a successful career in Medical Laboratory Science. Utilizing online resources like MLSi Academy and PASSASCP.COM can provide you with the tools and community support needed to thrive in this dynamic field.


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  2. “National Accrediting Agency for Clinical Laboratory Sciences.” NAACLS,
  3. “, Laboratory Continuing Education.” LabCE,
  4. “ASCP Exam Study Strategies.” MLSI Academy,
  5. “Study for the ASCP MLS.” MLSI Academy,
  6. “Board of Certification.” American Society for Clinical Pathology,
  7. “ASCP BOC Study Guide (5th Edition).” ASCP Store,
  8. “ASCP Preparation.” MLSI Academy,
  9. “MLT ASCP Exam.” Reddit – r/medlabprofessionals,
  10. “Medical Laboratory Science Practice Test.” Mometrix Test Preparation,
  11. “BOC Exam Prep.” ASCP,
  12. “CLS Resource.” CLS Resource,
  13. “PassASCP.” PassASCP,
  14. “Medical Laboratory Scientist Certification.” MLSI Academy,
  15. “MLSI Academy.” MLSI Academy,
  16. “YouTube Video on ASCP Preparation.” YouTube,
  17. “MLS Certification.” MLSI Academy,
  18. “How Hard Is the ASCP Exam Really?” Reddit – r/medlabprofessionals,
  19. “YouTube Video on ASCP Exam Preparation.” YouTube,

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