AMT MLS Domain 2: Chemistry Welcome to your AMT MLS Domain 2: Chemistry 1. AMT MLS: Chemistry In gas chromatography, what is the primary purpose of the carrier gas? To provide a medium for the sample to interact with the stationary phase To react chemically with the sample To detect the components of the sample To transport the sample through the column None 2. AMT MLS: Chemistry Which of the following statements is true regarding the use of a spectrophotometer in clinical chemistry? It measures the concentration of a substance by detecting the amount of light absorbed by a solution. It identifies compounds based on their molecular weight. It is primarily used for measuring the pH of solutions. It separates compounds based on their charge. None 3. AMT MLS: Chemistry In the context of clinical chemistry, what is the significance of the anion gap? It is a measure of the charge balance between cations and anions in the blood. It determines the pH level of the blood. It is used to assess kidney function. It indicates the oxygen-carrying capacity of red blood cells. None 4. AMT MLS: Chemistry What is the principle of flame emission spectroscopy? It measures the intensity of molecular vibrations. It detects the emission of light by atoms or ions in a flame. It quantifies the heat absorption by molecules. It identifies substances based on their crystalline structure. None 5. AMT MLS: Chemistry Which of the following techniques is used in clinical chemistry to separate proteins based on their size and electrical charge? High-performance liquid chromatography 'HPLC' Electrophoresis Mass spectrometry Flame photometry None 6. AMT MLS: Chemistry In clinical chemistry, what is the primary purpose of using a buffer solution in a biochemical assay? To stabilize the pH of the solution To increase the reaction rate To act as a catalyst in the reaction To isolate specific molecules from a mixture None 7. AMT MLS: Chemistry What is the clinical significance of measuring lactate dehydrogenase (LDH) activity in the blood? It is specific for diagnosing liver diseases. It is an indicator of myocardial infarction. It measures the oxygen-carrying capacity of blood. It is used to determine blood glucose levels. None 8. AMT MLS: Chemistry In mass spectrometry, what does the m/z ratio represent? Mass to charge ratio of ions Molecular weight of the compound Intensity of the ion signal Concentration of the analyte in the sample None 9. AMT MLS: Chemistry Why is the enzyme-linked immunosorbent assay 'ELISA' significant in clinical chemistry? It determines the molecular weight of proteins. It identifies the presence of specific ions in a solution. It quantifies specific proteins or antibodies in a sample. It measures the pH level of bodily fluids. None 10. AMT MLS: Chemistry In clinical chemistry, what is the significance of measuring serum creatinine? It assesses carbohydrate metabolism. It evaluates lipid profiles. It indicates renal function. It measures blood pH. None 11. AMT MLS: Chemistry What is the principle of the biuret method for protein determination? It measures the rate of protein hydrolysis. It detects the presence of peptide bonds in proteins. It quantifies proteins based on their aromatic amino acid content. It separates proteins based on their molecular weight. None 12. AMT MLS: Chemistry In the context of clinical chemistry, why is it important to measure serum electrolytes like sodium and potassium? To assess nutritional status To determine blood alcohol level To evaluate fluid and electrolyte balance To identify the presence of metabolic waste products None 13. AMT MLS: Chemistry What is the clinical relevance of measuring blood urea nitrogen (BUN)? It is a marker for protein intake and renal excretory function. It indicates the rate of hemoglobin synthesis. It assesses the buffering capacity of blood. It measures the concentration of blood glucose. None 14. AMT MLS: Chemistry How does high-performance liquid chromatography 'HPLC' differ from traditional column chromatography? HPLC uses a liquid mobile phase exclusively. HPLC provides higher resolution and faster analysis. HPLC is used only for organic compounds. HPLC requires larger sample volumes. None 15. AMT MLS: Chemistry What is the purpose of using a reference standard in quantitative assays? To provide a medium for the reaction To control the temperature of the assay To compare the test result for calibration To increase the reaction rate None 16. AMT MLS: Chemistry What is the role of a catalyst in a chemical reaction within the clinical chemistry laboratory? To increase the reaction rate without being consumed To act as a reactant in the reaction To decrease the product yield To stabilize the reactants None 17. AMT MLS: Chemistry In clinical chemistry, the measurement of what substance is crucial for diagnosing diabetes mellitus? Serum albumin Blood urea nitrogen (BUN) Blood glucose Serum bilirubin None 18. AMT MLS: Chemistry Why is ion-selective electrode (ISE) technology important in clinical chemistry? It measures the viscosity of biological fluids. It provides specific measurement of ion concentrations. It determines the molecular weight of proteins. It identifies the presence of organic compounds. None 19. AMT MLS: Chemistry In the context of enzymatic assays in clinical chemistry, what is the significance of the Michaelis-Menten constant (Km)? It indicates the maximum reaction rate. It determines the enzyme's affinity for its substrate. It measures the enzyme concentration. It calculates the final product yield. None 20. AMT MLS: Chemistry What is the principle behind turbidimetry in clinical chemistry? Measuring the light emitted by a sample Measuring the light absorbed by a sample Measuring the light scattered by a sample Measuring the fluorescence of a sample None 1 out of 20 Time is Up! Time's up
